Journal: The Journal of nutrition

Article Title: Mango Supplementation Modulates Gut Microbial Dysbiosis and Short-Chain Fatty Acid Production Independent of Body Weight Reduction in C57BL/6 Mice Fed a High-Fat Diet.

doi: 10.3945/jn.115.226688

Figure Lengend Snippet: FIGURE 2 Protein expression of pancreatic in- cretin receptors in C57BL/6 mice fed a control diet or an HF diet supplemented with 0%, 1%, or 10% mango for 12 wk. GLP1R (A) and GIPR (B) protein expression were determined via immunoblot anal- ysis (normalized to b-actin) in the pancreas of mice after 12 wk of dietary treatment. Data are means 6 SEMs (n = 5 mice/group). Labeled means without a common letter differ, P # 0.05. C, control; GIPR, gastric inhibitory peptide receptor; GLP1R, glucagon- like peptide 1 receptor; HF, high fat; HF+1%M, high fat + 1% mango; HF+10%M, high fat + 1% mango.

Article Snippet: Total protein (20 mg) was electrophoretically separated by use of SDS-PAGE and transferred to PVDF membranes (ThermoScientific), and membranes were blocked with 5% nonfat dried milk (Nestle) followed by overnight incubation at 4 C with the following rabbit polyclonal antibodies: GLP1R and GIPR (Santa Cruz Biotechnology) and b-actin (Cell Signaling Technology).

Techniques: Expressing, Control, Western Blot, Labeling